간행물

생화학분자생물학회입니다.


EMM

Biological importance of OCT transcription factors in reprogramming and development

  • 작성자

    관리자
  • 작성일자

    2021-07-07
  • 조회수

    192
Hans R. Schöler ( office-schoeler@mpi-muenster.mpg.de )
Since 2004 Director of Max Planck Institute for Molecular Biomedicine, Department for Cell and Developmental Biology, Münster, Germany
Since 2004 Professor of the Medical Faculty of the Westphalian Wilhelms-University Münster, Germany
Since 2014 Distinguished Professor at the Konkuk University, Seoul, South Korea
Since 2004 Adjunct Professor of Biochemistry, University of Pennsylvania, Center for Animal Transgenesis and Germ Cell Research, Philadelphia, USA
1999-2004 Professor and Marion Dilley and David George Jones Chair in Reproduction Medicine at the University of Pennsylvania, School of Veterinary Medicine, Department of Animal Biology in Philadelphia, USA
1994 Habilitation at the School of Biology at the Ruprecht-Karls-University in Heidelberg, Germany (venia legendi in Molecular Biology)
1991-1999 Head Research Group at the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany
1988-1991 Staff Scientist at the Max Planck Institute for Biophysical Chemistry in Göttingen, Germany
1986-1988 Head of Research Group at Boehringer Mannheim (now Roche) in Tutzing, Germany
1982-1985 Center for Molecular Biology Heidelberg (ZMBH) in Heidelberg, Germany (1985 PhD)
1982 Studies of Biology at the Ruprecht-Karls-University in Heidelberg, Germany (1982 diploma)

Biological importance of OCT transcription factors in reprogramming and development

Ectopic expression of Oct4, Sox2, Klf4 and c-Myc can reprogram somatic cells into induced pluripotent stem cells (iPSCs). Attempts to identify genes or chemicals that can functionally replace each of these four reprogramming factors have revealed that exogenous Oct4 is not necessary for reprogramming under certain conditions or in the presence of alternative factors that can regulate endogenous Oct4 expression. For example, polycistronic expression of Sox2, Klf4 and c-Myc can elicit reprogramming by activating endogenous Oct4 expression indirectly. Experiments in which the reprogramming competence of all other Oct family members tested and also in different species have led to the decisive conclusion that Oct proteins display different reprogramming competences and species-dependent reprogramming activity despite their profound sequence conservation. We discuss the roles of the structural components of Oct proteins in reprogramming and how donor cell epigenomes endow Oct proteins with different reprogramming competences.

Exp Mol Med. 2021 Jun 11. doi: 10.1038/s12276-021-00637-4. Online ahead of print.
https://pubmed.ncbi.nlm.nih.gov/34117345/