생화학분자생물학회

	Taekjip Ha ( taekjip.ha@childrens.harvard.ed )
	2023.08-present	George D. Yancopoulos Professor of Pediatrics in honor of Frederick W. Alt, Faculty affiliate, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School
	2025.01-present	Director, Program in Cellular and Molecular Medicine, Boston Children’s Hospital
	2023.08-present	Senior Investigator of Program in Cellular and Molecular Medicine, Boston Children’s Hospital
	2005.09–present	Investigator, Howard Hughes Medical Institute
	2015.08-2023.07	Bloomberg Distinguished Professor of Biophysics & Biophysical Chemistry, Biophysics and Biomedical Engineering, Johns Hopkins University
	2012.01–2015.08	Edward William and Jane Marr Gutgsell Endowed Professor, University of Illinois at Urbana-Champaign
	2008.09–2015.08	Co-director, Center for the Physics of Living Cells, University of Illinois
	2007.08–2015.08	Professor, Department of Physics and Center for Biophysics and Computational Biology, University of Illinois
	2004.08–2007.08	Associate Professor, Department of Physics and Center for Biophysics and Computational Biology, University of Illinois
	2000.08–2004.07	Assistant Professor, Department of Physics and Center for Biophysics and Computational Biology, University of Illinois
1998.01–2000.08	Postdoctoral Fellow (advisor: Steven Chu), Physics Dept, Stanford University
1997.01–1997.12	Postdoctoral Fellow (advisors: Shimon Weiss and Daniel Chemla), Lawrence Berkeley National Laboratory
1996.12	Ph.D. in Physics, University of California at Berkeley, Berkeley, CA 94720 Thesis: Fluorescence Spectroscopy of Single Molecules at Room Temperature and Its Applications (advisors: Shimon Weiss and Daniel Chemla)
1990.02	B.S. in Physics, Seoul National University, Seoul, Republic of Korea

Unveiling the invisible genomic dynamics

CRISPR-based imaging technologies have emerged as powerful tools for visualizing specific genomic loci, providing groundbreaking insights into chromatin structure and dynamics. Here, in this Review, we discuss the development and recent advances in these techniques, highlighting key strategies such as signal amplification, background reduction, multiplexing and enhanced genomic resolution. By engineering Cas proteins and guide RNAs, and incorporating peptide and aptamer tags, researchers have remarkably improved the sensitivity, specificity and resolution of CRISPR-based imaging, enabling the detection of nonrepetitive genomic regions and single-nucleotide polymorphisms. Recent studies have further pushed the boundaries of CRISPR-based imaging with the introduction of degron-mediated fluorogenic labeling and light-controllable background reduction. Despite remaining challenges, such as the bulkiness of signal amplification systems, limitations in guide RNA design and the effects of fixation on chromatin-protein interactions, CRISPR-based imaging holds great promise for advancing our understanding of chromatin dynamics, genomic interactions and their roles in various biological processes.

Exp Mol Med. 2025 Jul;57(7):1400-1408. https://doi.org/10.1038/s12276-025-01434-z
https://pubmed.ncbi.nlm.nih.gov/40745003/