생화학분자생물학회

	Seung Woo Cho ( swcho@unist.ac.kr )
	2019-present	Assistant Professor, Department of Biomedical Engineering, Ulsan National Institute of Science and Technology, South Korea
	2014-2018	Postdoctoral Researcher, Program in Epithelial Biology, Department of Dermatology, Stanford University School of Medicine, US
	2007-2014	MS/PhD, Department of Chemistry, Seoul National University, South Korea
	2001-2007	BS, Department of Chemistry, Choong-Ang University, South Korea

Applications of multiplexed CRISPR-Cas for genome engineering

The CRISPR-Cas system has become a worldwide genome editing tool for various organisms. Its precision and efficiency have facilitated basic research, drug discovery and therapeutic interventions. In contrast to other genome editing agents, CRISPR-Cas is modulated by a short guide RNA. Due to its simplicity, CRISPR-Cas is recognized as the best candidate for multiplexed genome editing. With simultaneous targeting, efficient knockout of genes with large deletions is possible. In addition, CRISPR-Cas can induce complex structural variations, such as inversions, translocations and duplications. Moreover, by utilizing engineered CRISPR-Cas proteins specialized for direct repression or activation of gene expression, one can perform multiplexed epigenetic editing. Lastly, multiplexed targeting enables killing of specific types of cells by accumulating stress mediated by simultaneous DNA damages. Here we discuss how CRISPR-based editing technologies for multiple targets are applied in recent studies.

Exp Mol Med. 2025 Jul;57(7):1373-1380. https://doi.org/10.1038/s12276-025-01500-6.
https://pubmed.ncbi.nlm.nih.gov/40745004/