생화학분자생물학회

	Name: Tae-Jin Kim ( tjkim77@pusan.ac.kr )
	2021-present	Associate Professor, Department of Biological Sciences, Pusan National University
	2017-2021	Assistant Professor, Department of Biological Sciences, Pusan National University
	2015-2017	Senior Researcher, Korea Institute of Science and Technology (KIST)
	2014-2015	Postdoctoral research fellow, Univ. of Washington at Seattle, USA
	2007-2013	Ph.D., Neuroscience, Univ. of Illinois at Urbana-Champaign, USA

Fluorescence-based techniques for investigating estrogen receptor dynamics

Understanding the signaling pathways of estrogen receptors (ER) is critical for revealing the intricate mechanisms behind diseases linked to estrogen, such as breast cancer, and addressing the adverse effects of environmental estrogenic disruptors. Traditionally, ER signaling involves a series of genomic events: ligand binding, receptor dimerization, and transcriptional modulation within the nuclei of cells. However, recent advances have highlighted the involvement of ERs in non-genomic signaling pathways, adding complexity to their functional roles. Researchers utilize sophisticated methods to thoroughly examine these pathways, particularly fluorescence-based techniques. These techniques employ a fluorescent probe (FPb) to visualize and monitor the dynamics of ER within living cells, shedding light on their spatial distribution, expression kinetics, and functional activities. A comprehensive examination of each aspect of ER signaling is essential to fully grasp the complexities involved. This review methodically organizes and explores the crucial features of fluorescent probes used to study various physiological aspects of ER signaling, including visualization of ER, ligand-receptor interactions, receptor dimerization, ERE-mediated transcriptional activation, and GPER signaling. The aim of this review is to provide valuable insights to researchers who plan to utilize FPb in their investigations of ER signaling.


BMB Rep. 2024 Sep 2:6277. Online ahead of print.
https://pubmed.ncbi.nlm.nih.gov/39219049/