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Single-molecule DNA-flow stretching assay as a versatile hybrid tool for investigating DNA-protein interactions

  • 작성자

    Sadaf Shehzad
  • 작성일자

    2024-12-26
  • 조회수

    412
Name: Sadaf Shehzad ( sadafshehzad@icgeb.res.in )
2024-presentProject scientist I, International Centre for Genetic Engineering and Biotechnology, India
2022-2023Postdoctoral fellow, Department of Physics and Astronomy, The University of Texas Rio Grande Valley, USA
2016-2022Ph.D., International Centre for Genetic Engineering and Biotechnology, India
Name: HyeongJun Kim ( hyeongjun.kim@utrgv.edu )
2024-presentAssociate Professor, Department of Physics and Astronomy, The University of Texas Rio Grande Valley, USA
2018-2024Assistant Professor, Department of Physics and Astronomy, The University of Texas Rio Grande Valley, USA
2011-2017Postdoctoral research fellow, Harvard Medical School, USA
2005-2011Ph.D., Department of Physics, The University of Illinois at Urbana-Champaign, USA

Single-molecule DNA-flow stretching assay as a versatile hybrid tool for investigating DNA-protein interactions

Single-molecule techniques allow researchers to investigate individual molecules and obtain unprecedented details of the heterogeneous nature of biological entities. They play instrumental roles in studying DNA-protein interactions due to the ability to visualize DNA or proteins and to manipulate individual DNA molecules by applying force or torque. Here, we describe single-molecule DNA-flow stretching assays as hybrid tools that combine forces with fluorescence. We also review how widely these assays are utilized in elucidating working mechanisms of DNA-binding proteins. Additionally, we provide a brief explanation of various efforts to prepare DNA substrates with desired internal protein-binding sequences. More complicated needs for DNA-protein interaction research have led to improvements in single-molecule DNA flow-stretching techniques. Several DNA flow-stretching variants such as DNA curtain, DNA motion capture assays, and protein-induced fluorescence enhancement (PIFE) are introduced in this mini review. Single-molecule DNA flow-stretching assays will keep contributing to our understanding of how DNA-binding proteins function due to their multiplexed, versatile, and robust capabilities.


BMB Rep. 2024 Dec 20. pii: 6382. [Epub ahead of print]
https://pubmed.ncbi.nlm.nih.gov/39701027/