생화학분자생물학회

	Sungchul Hohng ( shohng@snu.ac.kr )
	2015 - present	Professor, Department of Physics and Astronomy, Seoul National University
	2010 - 2015	Associate Professor, Department of Physics and Astronomy, Seoul National University
	2006 - 2010	Assistant Professor, Department of Physics and Astronomy, Seoul National University
	2002 - 2006	Postdoctoral Research Associate, Department of Physics, University of Illinois at Urbana-Champaign
	2000 - 2001	Postdoctoral Research Associate (mentor: Dai-Sik Kim), Department of Physics, Seoul National University, Korea
	1996 - 2000	Ph.D. in Physics, Seoul National University, Korea
	1994 - 1996	M.S. in Physics, Seoul National University, Korea
	1989 - 1994	B.S. in Physics, Seoul National University, Korea.

Analytical Techniques for Nucleic Acid and Protein Detection with Single-Molecule Sensitivity

Nucleic acids and proteins serve as crucial biomarkers used in the diagnosis, prognosis, and therapy monitoring across various diseases. Traditionally, these biomarkers are identified at an ensemble level following the amplification of target or signaling molecules. However, these methods have limitations in addressing contemporary challenges in molecular diagnostics, such as low sensitivity, specificity, throughput, and imprecise quantification. To overcome these limitations, various analytical techniques offering single-molecule sensitivity have been developed. This review aims to provide a concise overview of historically significant and potentially promising analytical techniques to detect nucleic acids and proteins with single-molecule sensitivity. Our focus is on their potential in liquid biopsy, delineating their strengths and weaknesses, providing insights into their ability to revolutionize biomarker analysis, and paving the way for more advanced technologies.

Exp Mol Med. 2025 May;57(5):938-949. https://doi.org/10.1038/s12276-025-01453-w
https://pubmed.ncbi.nlm.nih.gov/40307572/