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BmB Reports

Advanced principles and technologies in higher order chromatin architecture: move towards the 4D genome

  • 작성자

    Namyoung Jung
  • 작성일자

    2021-07-15
  • 조회수

    2
Name: Namyoung Jung ( jnamyoun@postech.ac.kr )
2020-present Postdoctoral research fellow, Department of Life Science, Pohang University of Science and Technology (POSTECH)
2016-2020 Postdoctoral research fellow, Department of Dermatology, Stanford University School of Medicine, USA
2009-2015 Ph.D., Cellular and molecular Medicine, Johns Hopkins University School of Medicine, USA
Name: Tae-Kyung Kim ( tkkim@postech.ac.kr )
2018-present Associate Professor, Department of Life Science, Pohang University of Science and Technology (POSTECH)
2010-2018 Assistant/Associate Professor, Department of Neurosciences, University of Texas Southwestern Medical Center, USA
2002-2009 Postdoctoral research fellow, Department of Neurobiology, Harvard Medical School
1993-2001 Ph.D., Department of Biochemistry, Rutgers University Medical School, USA

Advanced principles and technologies in higher order chromatin architecture: move towards the 4D genome

In eukaryotes, the genome is hierarchically packed inside the nucleus, which facilitates physical contact between cis-regulatory elements (CREs), such as enhancers and promoters. Accumulating evidence highlights the critical role of higherorder chromatin structure in precise regulation of spatiotemporal gene expression under diverse biological contexts including lineage commitment and cell activation by external stimulus. Genomics and imaging-based technologies, such as Hi-C and DNA fluorescence in situ hybridization (FISH), have revealed the key principles of genome folding, while newly developed tools focus on improvement in resolution, throughput and modality at single-cell and population levels, and challenge the knowledge obtained through conventional approaches. In this review, we discuss recent advances in our understanding of principles of higher-order chromosome conformation and technologies to investigate 4D chromatin interactions.


BMB Rep 2021 May;54(5):233-245. doi: 10.5483/BMBRep.2021.54.5.035.
https://pubmed.ncbi.nlm.nih.gov/33972012/