생화학분자생물학회

	Name: Chan Hyuk Kim ( Kimchanhyuk@kaist.ac.kr )
	2016-present	Assistant Professor, Department of Biological Sciences, Korea Advanced Institute of Science and Technology
	2012-2016	Principal Investigator, Department of Biology, California Institute for Biomedical Research, USA
	2009-2012	Postdoctoral research fellow, Scripps Research, USA
	2004-2008	Ph.D., Department of Chemistry, Seoul National University

Genome editing of immune cells using the CRISPR/Cas system

The ability to read, write, and edit genomic information in living organisms can have a profound impact on research, health, economic, and environmental issues. The CRISPR/Cas system, recently discovered as an adaptive immune system in prokaryotes, has revolutionized the ease and throughput of genome editing in mammalian cells and has proved itself indispensable to the engineering of immune cells and identification of novel immune mechanisms. In this review, we summarize the CRISPR/Cas9 system alongside the history of its discovery and optimization. We then focus on engineering T cells and other types of immune cells with emphasis on therapeutic applications. Lastly, we describe the different modifications of Cas9 and their recent applications in the genome-wide screening of immune cells.


BMB Rep 2020 Dec 11;5226. Online ahead of print.
https://pubmed.ncbi.nlm.nih.gov/33298251/