생화학분자생물학회

	Name: Cherlhyun Jeong ( che.jeong@kist.re.kr )
	2021-present	Principal Research Scientist, Chemical & Biological Integrative Research Center, KIST
	2012-2021	Senior Research Scientist, Center for Theragnosis, KIST
	2007-2011	Postdoctoral research fellow, Department of Physics, POSTECH
	2000-2007	Ph.D., Department of Electrical Engineering, Seoul National University
	Name: Jeongmin Lee ( jmin_105@korea.ac.kr )
	2020–Present	Unified M.S & Ph.D. course, Department of Life Sciences (Molecular Biology), Korea University, Republic of Korea
	2014–2020	B.S. in Fine Chemistry, Seoul National University of Science and Technology, Republic of Korea

Single-molecule perspectives of CRISPR/Cas systems: target search, recognition, and cleavage

CRISPR/Cas systems have emerged as powerful tools for gene editing, nucleic acid detection, and therapeutic applications. Recent advances in single-molecule techniques have provided new insights into the DNA-targeting mechanisms of CRISPR/Cas systems, in particular, Types I, II, and V. Here, we review how single-molecule approaches have expanded our understanding of key processes, namely target search, recognition, and cleavage. Furthermore, we focus on the dynamic behavior of Cas proteins, including PAM site recognition and R-loop formation, which are crucial to ensure specificity and efficiency in gene editing. Additionally, we discuss the conformational changes and interactions that drive precise DNA cleavage by different Cas proteins. This mini review provides a comprehensive overview of CRISPR/Cas molecular dynamics, offering conclusive insights into their broader potential for genome editing and biotechnological applications.


BMB Rep. 2024 Dec 20. pii: 6391. [Epub ahead of print]
https://pubmed.ncbi.nlm.nih.gov/39701024/